Solubility, thermodynamic

Thermodynamic solubility of the compound is investigated by incubating the solid compound in buffer at room temperature, the sample is filtrated and semi-quantitatively analyzed by UPLC/PDA against calibration curve.
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Test concentrations: 1

Time points: 18 h

Replicates: 2

UPLC/PDA analysis

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Thermodynamic solubility

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Excel report
(full report available per request)

Background

Only the dissolved fraction of a compound can move through membranes by diffusion or via transporter mechanisms or interact with receptors/enzymes. Solubility is an important parameter in drug development as it is limiting factor for the free-to-interact fraction of the compound in the gastrointestinal fluids, plasma and other matrices in the body. The solubility of a study compound is determined using either the traditional “shake-flask” method (thermodynamic solubility) or by spiking DMSO-stock solution into buffer-system (“kinetic” solubility) to minimize the consumption of the study compound. The samples will be filtrated and analyzed by UPLC/PDA/MS.

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