Released N-glycan profiling
Released N-glycan profiling. Enzymatic release of N-glycans, labelling of the released glycans with a fluorophore and UHPLC-FLD/MS analysis of the labelled glycans.
Background
Glycosylation is one of the most frequent protein post-translational modifications, which may affect the stability, immunogenicity, pharmacokinetics and biological activity of the protein. Therefore, comprehensive characterization and monitoring of glycans is an essential task in development of biologics. Characterization of released glycans provides more comprehensive information about the glycan profile in comparison to analysis of the intact glycoprotein.
