Protein MW and structural characterization

Determination of molecular weight for intact protein. Analysis using LC-HR/MS after removal of PEG using commercial detergent removal matrix.Intact molecular weight analysis of protein by mass spectrometry is a fast and efficient way to confirm purity, homogeneity and molecular weight of the protein analyte. The samples are analyzed using high resolution LC/MS system. In the case of antibodies, various treatments can be applied to verify the molecular weights of heavy and light chains of the antibody or other enzymatically produced sub-units, to enable more precise characterization of the target molecule. In addition, the effect of different glycosylations or other post-translational modifications on the molecular weight can be observed.