Permeability, PAMPA

The in vitro permeability of the test compounds are investigated using parallel artificial membrane permeation assay (PAMPA) with a filter membrane coated with gastrointestinal tract lipid formulation.
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Test concentrations: 1

Time points: 4h

Replicates: 3

Positive controls

UV-vis analysis

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Permeability value

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Excel report

Background

As a high-throughput screening permeability assay, we offer the Parallel Artificial Membrane Permeability Assay (PAMPA). This assay is used as an in vitro model for passive, transcellular permeation. It is a fast and easy method which determines the permeability of test compounds from a donor compartment, through a lipid-infused artificial membrane into an acceptor compartment. PAMPA studies are performed using the PAMPA explorer kit (pION Inc) and the double sink protocol.

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