Permeability, Caco-2

Apparent permeability of the test compound through Caco-2 cell monolayers in one or two directions is investigated using transwell plates. Cell monolayer integrity is controlled with transepithelial electrical resistance (TEER) measurement prior to the incubations and with Lucifer Yellow (LY) permeability measurement after the incubations.
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Test concentrations: 1

Time points: 0, 2h

Replicates: 2

Control compounds

UPLC/MS/MS analysis

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Apparent permeability

Recovery

Efflux ratio (in bi-directional assay)

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Excel or Full Word/PDF report

Background

The use of Caco-2 cells (human colon carcinoma cell line) has become the industry standard for investigation of permeation, as these cells endogenously express a variety of transporter proteins that are present in various endothelial and epithelial barriers of the body. In addition to Caco-2, we also provide MDCK (Madin Darby canine kidney cell line) assays for investigating permeation. Caco-2 and MDCK assays are available as mono- and bidirectional, and in the presence or absence of pH gradient across the cell monolayer. These experiments are intended for evaluation of epithelial and endothelial permeability, intestinal absorption, and involvement of active transport.

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