In vivo BBB permeability

The study formulations are dosed to study animals (mouse or rat) and plasma, brain and optionally CSF samples are collected and analyzed by UPLC/MS/MS.
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Test concentrations:

Time points: 8 for plasma, 3 for brain and optionally CSF

Replicates: 3

UPLC/MS/MS analysis

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Plasma, brain & CSF concentrations, PK calculations (e.g., brain-to-plasma ratio, CL, Vd, Cmax, Tmax, AUC, t1/2)

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Excel report
(full report available per request)

Background

In in vivo DMPK studies, compounds are administered to rodents and samples collected over a pre-defined time period. The dosing regimen and sampling are designed according to the project needs and prior information available on the study compounds, and the concentration-time data can be summarized using non-compartmental analysis (NCA) or compartmental modelling. Several options are available for bioanalytics, and the methods are always optimized for the study compounds instead of using generic methods. Furthermore, when LC/HR-MS is used, the same data can be used later for metabolite identification and profiling.

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