Hepatotoxicity (DILI)

Using pooled primary human hepatocytes and automated high-content imaging tool, the compound is tested whether it induces hepatotoxicity & cell stress, steatosis & phospholipidosis and/or accumulates into lysosomes.
check Protocol

Test concentrations: 3 or 6

Time points: 1

Replicates: 3

Positive controls

Automated fluorescence microscope analysis

check Deliverables

Fold change compared to vehicle control
Decrease in lysosomal staining, % of vehicle control

check Reporting

Excel report
(full report available per request)

Background

Drug-induced liver failure, DILI, is one of the major causes of acute liver failure as a result of direct toxicity from the administered drug or its metabolites. Therefore, it is essential to address hepatotoxicity risks early in the drug discovery process. Hepatotoxicity can be induced by different mechanisms such as induction of fat accumulation in the liver or disturbances in the normal lysosomal function of hepatocytes.

More details chevron_right