Genotoxicity, MNT
The test compound is investigated for its ability to cause chromosomal aberrations or breakages by the in vitro micronucleus test MNT utilizing flow cytometry as a detection method.
Test concentrations: 11
Time points: 4h/ +S9 exposure followed by 20h recovery time and 24h/ -S9 exposure
Replicates: 2
Positive controls
Flow cytometry analysis
Background
AMES test is used to identify compounds that can cause spontaneous point mutations such as base pair substitutions, deletions or duplications. These events can ultimately lead to cancer susceptibility. MNT assay is used to detect drug candidates causing chromosomal aberrations (aneugen) or breakages (clastogen) leading to perturbations in cell division. The resulting micronuclei adjacent to main nuclei are also a sign of genotoxicity.
