Genotoxicity, AMES

The test compound is tested for ability to cause mutagenesis by bacterial reverse mutation test, as two-strain (mini AMES) or five-strain (full AMES) AMES assay utilizing TA98, TA100, TA1535, TA1537 and E.coli strain WP2uvrA with and without PBN induced rat liver S9.
check Protocol

Test concentrations: 6

Time points: 48 h

Replicates: 3

Positive controls

Absorbance analysis

check Deliverables

Mutagenic events as fold induction over the baseline (fold induction higher than 2-fold and/or clear dose response may classify the compound mutagenic)

check Reporting

Excel or Full Word/PDF report

Background

AMES test is used to identify compounds that can cause spontaneous point mutations such as base pair substitutions, deletions or duplications. These events can ultimately lead to cancer susceptibility. MNT assay is used to detect drug candidates causing chromosomal aberrations (aneugen) or breakages (clastogen) leading to perturbations in cell division. The resulting micronuclei adjacent to main nuclei are also a sign of genotoxicity.

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