Cytotoxicity
Test concentrations: 3 or 7
Time points: 24 h
Replicates: 3
Positive control
Fluorescence & luminescence analysis
Background
Cytotoxicity (i.e., being toxic to cells) is almost by default an unwanted characteristic in drug discovery and development since cytotoxic compounds could have serious adverse effects. In addition, results of cytotoxicity assay can be used to guide testing concentrations of other assays, e.g. drug interaction studies to avoid overt toxicity. Fortunately, cytotoxicity can be studied by several in vitro methods based on measuring cell viability or cell death.
