Blood to plasma ratio
The test compound is incubated in whole blood and plasma and the samples are analyzed by using UPLC/MS/MS method developed for the purpose to determine the blood to plasma ratio.
Test concentrations: 1
Time points: 10 + 60 min
Replicates: 3
Positive control
UPLC/MS/MS analysis
Background
Binding to red blood cells affects the partitioning of the compound between blood and plasma and may lead to high blood to plasma ratio. If not taken into account, high blood to plasma ratio may result in incorrect calculation of the pharmacokinetic parameters as they are typically determined by analysis of compound concentrations in the plasma.
