Binding kinetics, SPR
Binding kinetics of test items (analytes) to solid phase bound target protein (ligand) are investigated using surface plasmon resonance.
Background
Surface plasmon resonance (SPR) and bio-layer interferometry (BLI) are label-free techniques which are used to investigate biomolecular interactions. They are especially suitable for characterizing antibody-antigen interactions and Fc-related antibody-effector interactions. In these techniques, the ligand is captured on the biosensor surface and binding of the analyte is investigated and information on the association and dissociation kinetics is obtained.