Mechanistic toxicity

Using HepG2 cells in monolayer, the test compound is tested for mitochondrial toxicity using Glu/Gal assay, oxidative stress using GSH/GSSG assay and/or caspase 3/7 activation.
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Test concentrations: 3 or 7

Time points: 1

Replicates: 3

Positive controls

Fluorescence analysis

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% of ATP (and cytotoxicity), compared to vehicle control in presence of glucose and galactose, respectively

Ratio of GSH/GSSG compared to vehicle control

Caspase 3/7 activation compared to vehicle control

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Excel report
(full report available per request)

Background

Mechanistic toxicity assays are an important part of our in vitro toxicology service portfolio, as they provide further information about the cell toxicity displayed by the study compound. All assays are with fluorescence or luminescence end-points.

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