Solubility, kinetic

Only the dissolved fraction of a compound can move through membranes by diffusion or via transporter mechanisms or interact with receptors/enzymes. Solubility is an important parameter in drug development as it is limiting factor for the free-to-interact fraction of the compound in the gastrointestinal fluids, plasma and other matrices in the body. The solubility of a study compound is determined using either the traditional “shake-flask” method (thermodynamic solubility) or by spiking DMSO-stock solution into buffer-system (“kinetic” solubility) to minimize the consumption of the study compound. The samples will be filtrated and analyzed by UPLC/PDA/MS.