CYP inhibition, hepatocytes
Test concentrations: 6
Time points: 1
Replicates: 2
Positive controls
UPLC/MS/MS analysis
Background
Inhibition of cytochrome P450 (CYP) enzymes by a new chemical entity (NCE) may decrease the metabolism of co-medicated drugs. We offer assays for individual enzymes as well as a cocktail approach to determine inhibition towards multiple enzymes in the same incubation. Liver microsomes are recommended for high-throughput screenings and for mechanistic approach. In addition, CYP inhibition evaluation can be conducted using pooled hepatocytes, for compounds where hepatocytes are a better suited model.